Isolation of Vacuoles from Saccharomyces Cerevisiae
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چکیده
Any proteomics analysis starts with sample preparation. For organelle proteomics, where the aim is the identification of organelle-specific proteins, the sample preparation involves the isolation of an organelle of interest to a high degree of purity devoid of cross-contaminations from other organelles. Different organelles of the yeast Saccharomyces cerevisiae possess unique physical/chemical properties that can be exploited or taken into account during isolation. The vacuole has a low density (it is the ‘lightest’ organelle of yeast) and can easily be separated from other organelles by density gradient centrifugation, yielding highly pure vacuole preparations that can be used for proteomics studies. II. CHAPTER: Isolation of vacuoles from S. cerevisiae
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